Archive for the ‘DNA Transfection Troubleshooting & Questions’ Category
Last Updated on Wednesday, 9 June 2010 12:50 Written by Administrator Wednesday, 9 June 2010 12:50
Question by Hil: Proper way to use the word transfected?
Hi, I think that I have heard that this term: transfected, transfect, transfection etc is supposed to be used in a certain way. Which of the following is correct? Or are they both correct? Thanks.
1. The plasmid DNA was transfected into HeLa cells.
2. The HeLa cells were transfected with plasmid DNA.
is it the same for transform? Please provide a source if you have one.
Best answer:
Answer by joanna j
“A first series of cellular models has been engineered by transfecting the ER? positive MCF-7 cell line with a plasmid expressing an inducible FLAG-tagged version of ER?”
So, the second one.
Transfection is a type of transformation, which is a general term meaning to alter a cell by introducing foreign DNA.
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Tags: Proper, transfected, word | Posted under DNA Transfection Troubleshooting & Questions | No Comments
Last Updated on Tuesday, 8 June 2010 12:29 Written by Administrator Tuesday, 8 June 2010 12:29
Question by David E: I am interested in going into forensics. Am I qualified for it?
I have a BSc in Biology with 6 years lab experience including Western Blots, Immunohistochemistry, PCR, Tissue Culture, ELISA, animal handling, radio-immuno assays – Binding/ Uptake, DNA transformation/ transfection, good sterile technique and have lots of experience working with hazardous chemicals/ biohazardous/ controlled substances.
I have good presentation skills and general lab skills. I am currently a research analyst principle, working for a university. I would like to work in forensics. What else should I be doing in order to get qualified – do I need to do a forensics degree specifically or is my experience sufficient.
Thanks in advance.
Best answer:
Answer by Kathy A
Dude, looks like u got all the qualifications, however do u have a strong stomach? Because u are going to need it.
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Tags: forensics., going, interested, into, qualified | Posted under DNA Transfection Troubleshooting & Questions | 3 Comments
Last Updated on Monday, 7 June 2010 09:02 Written by Administrator Monday, 7 June 2010 09:02
Question by AndroG: Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
To be more precized, I extracted the plasmid with my gene of interest from a bacteria with the special maxi kit endo-free from Qiagen and I eluted it with a buffer which contains 10mM Tris-C,pH8.0 and 1mM EDTA.I want to know how long can I store the eluted DNA at 4 degrees before using it for transfection into cells. Is DNA stable for several weeks or months? I would appreciate very much your answer as I am just a PhD student with not so much experience in molecular biology. Thank u very much.
Best answer:
Answer by clavdivs
No, but I could always send you a photo of a hamburger.
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Tags: 4dgr, anyone, eluted, know, many, solution, stable, TrisClEDTA, weeks/months | Posted under DNA Transfection Troubleshooting & Questions | 4 Comments
Last Updated on Thursday, 3 June 2010 01:25 Written by Administrator Thursday, 3 June 2010 01:25
Question by Kokk Kostas: Does anyone know how the Gene Jammer Transfection Reagent works?
We used GeneJammer Transfection Reagent in cells, before adding DNA. But I don’t know what it does exactly.
Best answer:
Answer by Nickname (exactly 32 characters)
The Gene Jammer Transfection Reagent contains a type of polycationic compound known as a polyamine. This compound is like a straight, stiff chain with several positively charged amino groups on it. Since DNA is a highly negatively charged molecule because of all the phosphate groups in its double helical backbone, the DNA becomes fluffed up or conditioned (kind of like if spaghetti strands were to move apart from each other because toothpicks separated all the spaghetti strands). This allows the target cell to more easily incorporate the DNA by endocytosis.
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Tags: anyone, gene, Jammer, know, Reagent, transfection, works | Posted under DNA Transfection Troubleshooting & Questions | No Comments