Archive for the ‘DNA Transfection Videos’ Category
Last Updated on Wednesday, 23 June 2010 06:04 Written by Administrator Wednesday, 23 June 2010 06:04
Research at the UW Medical Center translates to improving care options for patients. This University of Washington program shows what is going on in Dr. Clifford Hume,s lab, where researchers are exploring a biological approach to hearing restoration by stimulating regeneration of hair cells in the inner ear. To see more videos from the University of Washington visit uwtv.org.
Video Rating: 5 / 5
Tags: Hearing, Loss, Molecular, therapy | Posted under DNA Transfection Videos | 2 Comments
Last Updated on Monday, 21 June 2010 08:29 Written by Administrator Monday, 21 June 2010 08:29
I Love Gengineering (genetic engineering) Lyrics I’m nice right now I feel good If you have a culture Would you please put it in the air? That white sheep last night was awfully crazy I wish we cloned it Id take a nucleus and have this one egg completely naked Inject the genes into the egg and wait for the embryo to stage up Give it to mom, pop out a clone, name it Dolly and make it our own. Man, I love gengineering I wanna engineer plasmids the rest of my life Restriction enzymes and restriction sites Fragment DNA and put our gene in And then ligate it back up for the win So transfect my vector into bacteria But first make a ton using PCR-ia I am transfection champion Herbert Boyer, Stanley Cohen Don’t get excited, this is process is weak Those with Ab resistance are what we seek All others will perish and leave what we cherish Woke up today and all I could say is That white sheep last night was awfully crazy I wish we cloned it Id take a nucleus and have this one egg completely naked Inject the genes into the egg and wait for the embryo to stage up Give it to mom, pop out a clone, name it Dolly and make it our own. Man, I love gengineering, ay! And I love cloning, ay! I love plasmids, ay! Man, I love gengineering I can tell you what I learned from school and Gengineering is a powerful tool, um Yeah, of course I learned some facts Gene therapy and what it lack In your retrovirus your gene will pack No replication makes it a hack So another cell it will jack And the …
Tags: BlakeCarpenterNilashaGhosh2010StanfordE25 | Posted under DNA Transfection Videos | No Comments
Last Updated on Saturday, 19 June 2010 02:49 Written by Administrator Saturday, 19 June 2010 02:49
The ever talented, multi-faced Beth Barber agreed to help me out on this song. Music and Lyrics by yours truly. This song is sung between two professors: an arrogant male professor of molecular biology who whines about his inability to win the Nobel Prize and the hearts of young maidens (played by me) and a hippyish lady professor who has spent her life cleaning up after his destructive path (played by Beth). This song tells the story of his many misadventures coming up through the ranks of academe. His TA (also played by Beth) listens attentively. The lady professor, Anna Innana, is hiding in the bushes and overhears his tale and gives a response. Lyrics: Prof: I was just sure when I went into this field that I would quickly rise to the top and soon be flocked by beautiful young admirers. But somehow, it just never worked out that way. Oh the many hours that I spent pouring o’er my microscope Oh the prospects dour, and yet, faced with doubt, in spite I’d hoped That just one final chemical reaction Would finally lead to my long sought for satisfaction… I’ve always seemed to have trouble capitalizing on my superior intellect. Why I even remember back in college… I saw this girl in my class one day Let me tell ya’ pal — she was quite a gal I said “Would you like to talk DNA?” It was a real good line – it worked so very fine. We met up in the afternoon to study chapter six. I thought that she was sure to swoon — we’d be the next double helix! We talked transfection, we …
Video Rating: 5 / 5
Tags: Belle, Nobel, Prize | Posted under DNA Transfection Videos | No Comments
Last Updated on Sunday, 13 June 2010 04:35 Written by Administrator Sunday, 13 June 2010 04:35
Our vision at Aldevron is to be a world-class global contract services organization. To achieve this, we need to understand what our clients need and deliver what they want. One of our services is expression screening in insect cells. We offer a range of options to suite the clients needs. A typical screening project includes multiple variants, such as amino acid changes or truncations, of a single gene. We are able to screen multiple variants and culture conditions in parallel. There are a couple of options for expression screening in insect cells. The first option is to screen for expression via baculovirus infection. We can either start with a recombinant transfer vector supplied by the client or we can clone the gene of interest into a baculovirus transfer vector. Next, we generate a high-titer baculovirus stock and determine the viral titer. We then infect 10ml Sf21 cultures for recombinant protein expression. The second option is to screen for expression via direct transfection. This allows us to go from plasmid to protein in as little as 48 hours. We start by cloning the gene of interest into an expression vector designed to enable robust expression in insect cells without the need to produce recombinant baculovirus. Next we transfect 10ml Sf9 cultures with the recombinant plasmids. 48-72 hours post-transfection, we harvest the cells. Because generating a recombinant baculovirus can require anywhere between 2-4 weeks of time, the direct transfection method offers a …
Tags: Aldevron, cells, Expression, Insect, Protein | Posted under DNA Transfection Videos | No Comments