Archive for the ‘DNA Transfection Videos’ Category

Allele Biotech, now including Orbigen, provides services at all stages of the protein expression, production, and purification processes from gene cloning to large-scale fermentation. More than a decade ago, our technical staff offered the first commercial Baculovirus protein expression services to address researchers´ unmet gene expression needs. Today we are on the cutting edge of a variety of expression and antibody production technologies so that you can accelerate your research by taking advantage of our comprehensive range of services. In terms of protein expression, bacteria and yeast are commonly used to express a variety of proteins because they are more convenient and less costly than other systems. However, they may not be suitable for producing mammalian proteins with important post-translation modifications (PTM). Insect cells can be used effectively for producing large quantities of mammalian proteins rather easily through baculovirus such as Allele´s Sapphire system. PTM in insect cells is not exactly the same as in mammalian cells, eg different glycosylation patterns. Mammalian cells are used for proteins that require appropriate PTM or are not soluble in other systems through either transient transfection or stable cell line establishment. Allele Biotech is developing its own system to use baculovirus for expression in mammalian cells that will avoid high costs associated with large scale transient transfection, or the time consuming stable cell line …
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