Does anyone know how the Gene Jammer Transfection Reagent works?
Does anyone know how the Gene Jammer Transfection Reagent works?
Last Updated on Thursday, 3 June 2010 01:25 Written by Administrator Thursday, 3 June 2010 01:25
Question by Kokk Kostas: Does anyone know how the Gene Jammer Transfection Reagent works?
We used GeneJammer Transfection Reagent in cells, before adding DNA. But I don’t know what it does exactly.
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Answer by Nickname (exactly 32 characters)
The Gene Jammer Transfection Reagent contains a type of polycationic compound known as a polyamine. This compound is like a straight, stiff chain with several positively charged amino groups on it. Since DNA is a highly negatively charged molecule because of all the phosphate groups in its double helical backbone, the DNA becomes fluffed up or conditioned (kind of like if spaghetti strands were to move apart from each other because toothpicks separated all the spaghetti strands). This allows the target cell to more easily incorporate the DNA by endocytosis.
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