Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
Last Updated on Monday, 7 June 2010 09:02 Written by Administrator Monday, 7 June 2010 09:02
Question by AndroG: Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
To be more precized, I extracted the plasmid with my gene of interest from a bacteria with the special maxi kit endo-free from Qiagen and I eluted it with a buffer which contains 10mM Tris-C,pH8.0 and 1mM EDTA.I want to know how long can I store the eluted DNA at 4 degrees before using it for transfection into cells. Is DNA stable for several weeks or months? I would appreciate very much your answer as I am just a PhD student with not so much experience in molecular biology. Thank u very much.
Best answer:
Answer by clavdivs
No, but I could always send you a photo of a hamburger.
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Tags: 4dgr, anyone, eluted, know, many, solution, stable, TrisClEDTA, weeks/months
at 4C the DNA should be good at least for months maybe over a year if you keep it at -20C or -80C it will be good for many years
It should be quite stable for months. You wouldn’t have anything to lose by trying it. If it doesn’t work, you could always prepare a fresh batch of plasmid again.
I’ve shipped DNA at ambient temperature and had no problems, so 4 should definitely be OK for a while. But it is generally best to store your DNA preps at -20 (or even -80).
It ought to be stable for weeks or months, but it ought to be stored frozen. -20 ought to be fine. The EDTA in your TE ought to prevent degradation of your plasmid.