Posts Tagged ‘know’
Last Updated on Monday, 7 June 2010 09:02 Written by Administrator Monday, 7 June 2010 09:02
Question by AndroG: Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
To be more precized, I extracted the plasmid with my gene of interest from a bacteria with the special maxi kit endo-free from Qiagen and I eluted it with a buffer which contains 10mM Tris-C,pH8.0 and 1mM EDTA.I want to know how long can I store the eluted DNA at 4 degrees before using it for transfection into cells. Is DNA stable for several weeks or months? I would appreciate very much your answer as I am just a PhD student with not so much experience in molecular biology. Thank u very much.
Best answer:
Answer by clavdivs
No, but I could always send you a photo of a hamburger.
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Tags: 4dgr, anyone, eluted, know, many, solution, stable, TrisClEDTA, weeks/months | Posted under DNA Transfection Troubleshooting & Questions | 4 Comments
Last Updated on Thursday, 3 June 2010 01:25 Written by Administrator Thursday, 3 June 2010 01:25
Question by Kokk Kostas: Does anyone know how the Gene Jammer Transfection Reagent works?
We used GeneJammer Transfection Reagent in cells, before adding DNA. But I don’t know what it does exactly.
Best answer:
Answer by Nickname (exactly 32 characters)
The Gene Jammer Transfection Reagent contains a type of polycationic compound known as a polyamine. This compound is like a straight, stiff chain with several positively charged amino groups on it. Since DNA is a highly negatively charged molecule because of all the phosphate groups in its double helical backbone, the DNA becomes fluffed up or conditioned (kind of like if spaghetti strands were to move apart from each other because toothpicks separated all the spaghetti strands). This allows the target cell to more easily incorporate the DNA by endocytosis.
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Tags: anyone, gene, Jammer, know, Reagent, transfection, works | Posted under DNA Transfection Troubleshooting & Questions | No Comments