Posts Tagged ‘transfection’
Last Updated on Friday, 4 June 2010 08:40 Written by Administrator Friday, 4 June 2010 08:40
Tags: carcinoma, cell, following, fusion, gene, human, Identification, novel, oncogenic, ovarian, transfection | Posted under DNA Transfection Books | No Comments
Last Updated on Thursday, 3 June 2010 01:25 Written by Administrator Thursday, 3 June 2010 01:25
Question by Kokk Kostas: Does anyone know how the Gene Jammer Transfection Reagent works?
We used GeneJammer Transfection Reagent in cells, before adding DNA. But I don’t know what it does exactly.
Best answer:
Answer by Nickname (exactly 32 characters)
The Gene Jammer Transfection Reagent contains a type of polycationic compound known as a polyamine. This compound is like a straight, stiff chain with several positively charged amino groups on it. Since DNA is a highly negatively charged molecule because of all the phosphate groups in its double helical backbone, the DNA becomes fluffed up or conditioned (kind of like if spaghetti strands were to move apart from each other because toothpicks separated all the spaghetti strands). This allows the target cell to more easily incorporate the DNA by endocytosis.
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Tags: anyone, gene, Jammer, know, Reagent, transfection, works | Posted under DNA Transfection Troubleshooting & Questions | No Comments
Last Updated on Wednesday, 2 June 2010 09:31 Written by Administrator Wednesday, 2 June 2010 09:31
Question by mjorizzle: Why does transfection efficiency increase when the amount of DNA added increases in cells?
Best answer:
Answer by Michael P
The limiting step is getting DNA from outside to inside the cell. The more DNA there is, the more likely some molecules will slip through the membrane.
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Tags: added, amount, cells, efficiency, increase, increases, transfection | Posted under DNA Transfection Troubleshooting & Questions | 1 Comment
Last Updated on Tuesday, 1 June 2010 02:03 Written by Administrator Tuesday, 1 June 2010 02:03
Introduction to DNA transfection
Tags: Electroporation, Introduction, Mechanisms, Mirus, Optimization, Part, transfection | Posted under DNA Transfection Videos | 3 Comments