Posts Tagged ‘Transfer’
Last Updated on Saturday, 19 June 2010 08:21 Written by Administrator Saturday, 19 June 2010 08:21
Gene Transfer to Animal Cells (Advanced Methods)
The Adanced Methods series is intended for advanced undergraduates, graduate students and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems.
Gene transfer to animal cells was first achieved more than 30 years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells describes the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. The final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
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Last Updated on Thursday, 17 June 2010 01:07 Written by Administrator Thursday, 17 June 2010 01:07
DNA Transfer to Cultured Cells (Culture of Specialized Cells)
DNA transfer to cultured cells
Edited by Katya Ravid and R. Ian Freshney
Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics.
DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including:
* Viral infection
* Electroporation
* Phosphate precipitation
* DEAE Dextran
* Liposomes
* Yeast artificial chromosomes (YACs)
* Whole chromosome transfer
* Enhanced expression.
Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a unique and unparalleled resource.
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Tags: cells, Culture, Cultured, Specialized, Transfer | Posted under DNA Transfection Books | No Comments
Last Updated on Tuesday, 15 June 2010 03:06 Written by Administrator Tuesday, 15 June 2010 03:06
Lateral DNA Transfer: Mechanisms and Consequences
The Salk Institute, La Jolla, CA. Discusses how lateral transfer occurs, the extent to which it happens, and its implications for our understanding of biology. For biology students and researchers. Softcover, hardcover available.
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Tags: Consequences, Lateral, Mechanisms, Transfer | Posted under DNA Transfection Books | 1 Comment